practical Liver Function Tests

FUNCTIONS OF LIVER
CLASSIFICATION OF LIVER FUNCTION TESTS
Classification of LFT
• According to function of liver
• Tests based on synthetic function

• Tests based on excretory function

• Tests based on detoxification function

• Tests based on metabolic function

• Determination of serum enzymes

I. Tests based on excretory function
II. Tests b/o detoxification function
III. Tests b/o synthetic function
IV. Tests b/o metabolic function
• Tests related to CARBOHYDRATE metabolism
• Tests related to LIPID metabolism
• Tests related to PROTEIN metabolism



V. Enzymes in diagnosis of liver disease
I. Tests based on excretory function
I. Tests based on excretory function
I. Tests based on excretory function

Determination of Serum Bilirubin
• Serum bilirubin estimation is based on van den Berg’s diazo reaction.
• Diazo reagent consists of diazotized sulfanic acid
I. Tests based on excretory function
I. Tests based on excretory function
I. Tests based on excretory function
Laboratory results
I. Tests based on excretory function
Tests b/o detoxification function
Tests b/o synthetic function
• Liver is the main source of synthesis of
• Plasma proteins
• - Albumin
• - Globulin(except ?)
• Blood clotting factors
• - Prothrombin and
• factors V, VII and X.
•

Determination of serum albumin and globulin
Determination of Prothrombin time
Tests b/o metabolic function
• Tests related to CARBOHYDRATE metabolism
• Tests related to LIPID metabolism
• Tests related to PROTEIN metabolism



Enzymes in diagnosis of liver disease
• In liver cell injury, damage to the membrane of cells and organelles allows intracellular enzymes to leak into the blood.
• Where their elevated concentrations can be measured
Enzymes in liver disease
Serum transaminases