CHOLESTROL

CLINICAL SIGNIFICANCE
SAFETY CAUTIONS
(1) (2)
BIOLABO reagents are designated for professional, in vitro diagnostic
Total cholesterol assay, associated to assays of others lipids in serum
use.
is used in the diagnosis of hyperlipidemia. Increased levels are also
·
Verify the integrity of the contents before use.
seen in hepatic and thyroïd disorders.
·
Use adequate protections (overall, gloves, glasses).
Total cholesterol assay associated to triglycerides, HDL-Cholesterol
·
and LDL-Cholesterol determination is useful in the prediction of
Do not pipette by mouth.
coronary heart diseases.
·
In case of contact with skin and eyes, thoroughly wash affected
So this assay is used in the diagnosis and treatment of atherosclerotic
areas with plenty of water and seek medical advise.
diseases. Hypercholesterolemia can also be observed in certain cases
·
Reagents contain sodium azide (concentration < 0.1%) which may
of diabetus. Secondary disorders that elevate cholesterol levels,
react with copper and lead plumbing. Flush with plenty of water
should be ruled prior to initiating therapy with cholesterol-lowering
when disposing.
drugs.
·
Material Safety Data Sheet is available upon request.
·
Waste disposal: Respect legislation in force in the country.
All
specimens
should
be
handled
as
potentially
infectious,
in
PRINCIPLE
(4)
accordance
with
good
laboratory
practices
using
appropriate
Enzymatic method described by Allain and al., which reaction scheme
precautions. Respect legislation in force in the country.
is as follows:
REAGENTS PREPARATION
CE
Cholesterol esters
Cholesterol + free fatty acids
Add promptly the content of vial R2 (Enzymes), into vial R1 (Buffer).
Mix gently until complete dissolution (approximately 2 minutes).
CO
Cholesterol +
O2
Cholesten 4 one 3 +
H2O2
Vial R2:
If appropriate, use a non-sharp instrument to remove
POD
+
+
Quinoneimine (pink)
2
H2O2
Phenol
+ 4
H2O
PAP
aluminium cap.
STABILITY
REAGENTS COMPOSITION
Vial R1
BUFFER
Phosphate buffer
100
mmol/L
Chloro-4-phenol
5 mmol/L
Sodium Cholate
2.3
mmol/L
Triton x 100
1.5
mmol/L
Preservative
Vial R2
ENZYMES
Cholesterol oxydase (CO)
> 100
IU/L
Cholesterol esterase (CE)
> 170
IU/L
Peroxydase (POD)
> 1200
IU/L
4 - Amino – antipyrine (PAP)
0.25
mmol/L
PEG 6000
167
µmol/L
Vial R3
STANDARD
Cholesterol 200 mg/dL (5.17 mmol/L)