Mg

CLINICAL SIGNIFICANCE
(1)
Adult human body (70 Kg) contains 21 to 28 g of magnesium. Of this,
about 60% is in bone, 20% in skeletal muscle, 19% in other cells, and
about 1% in the extracellular fluids. About 30% of magnesium in
plasma is associated with proteins (primarily albumin). Consequently,
a change in the concentration in albumin can affect the concentration
in magnesium.
Hypomagnesaemia may be a secondary effect in hypocalcemic or
calcium-deficient tetany. Conditions that have been associated with
hypomagnesemia include chronic alcoholism, childhood malnutrition,
lactation, malabsorption, acute pancreatitis, hypothyroïdism, chronic
glomerulonephritis, aldosteronism, digitalis intoxication and prolonged
intravenous feeding.
Hypermagnesaemia have been observed in dehydratation, severe
diabetic acidosis, and immediately following myocardial infarction.
PRINCIPLE
(1) (4) (5)
Gindler, Heth and Khayam-Bashi method. Calmagite, a metallochromic
-[

indicator
(1 -[1-hydroxy-4-methyl-2-phenylazo]-2-naphtol-4-sulfonic
acid), forms in basic buffered medium a coloured complex with the
magnesium.
The
absorbance,
measured
at
510-550 nm,
is
proportional to the concentration of magnesium in the specimen.
EGTA reduces Calcium interference, Potassium cyanide (KCN)
reduces interference of heavy metals and surfactants reduce the
interference of proteins and lipemia.
REAGENTS COMPOSITION
Vial R1