calicium

CLINICAL SIGNIFICANCE
(1) (2)
Total Calcium exists in 3 physiochemical states in plasma, of which
approximately 50 % is free or ionised calcium, 40 % is bound to
plasma proteins, and 10 % are bound with small anions.
The
level
of
serum
calcium
may
be
affected
by
intestinal
malabsorption, by alterations in plasma proteins level, especially
albumin, which should be measured concurrently with calcium.
Hypercalcemia is found in hyperparathyroïdism, multiple myeloma,
bone
and
parathyroïdal
neoplasms
and
in
states
with
bones
demineralisation.
Hypocalcemia is encountered in hypoparathyroïdism and in several
cases of necrosis and acute pancreatitis.
PRINCIPLE
(4)
Moorehead and Briggs derived CPC (O-Cresol Phtalein Complexone)
method allows to determinate total Calcium concentration in serum,
plasma or urines.
In alkaline solution CPC reacts with calcium to form a dark-red
coloured complex which absorbance measured at
570 nm
is
proportional to the amount of calcium in the specimen