triglyceride

CLINICAL SIGNIFICANCE
(1)
The measurement of the concentration in blood triglycerides is
important for the diagnosis and the follow-up of hyperlipidemia. Its
increase can be of genetic origin or secondary to other metabolic
disorders such as: diabetes mellitus, hyper and hypothyroidisms,
hepatic diseases, acute and chronic pancreatitis, nephrosis. A rise in
triglycerides
also
represents
an
atherogenic
risk
factor.
It
is
responsible for the opalescence, or even the cloudiness of the serum.
Corticoids and oestrogen/progestin treatments can also aggravate
hypertriglyceridemia.
PRINCIPLE
The absorbance of the coloured complex (quinoneimine), proportional
to the amount of triglycerides in the specimen, is measured at 500 nm.
REAGENTS
Vial R1
BUFFER
PIPES
100
mmol/L
Magnesium chloride
9.8
mmol/L
Chloro-4-phenol
3.5
mmol/L
Preservative
Vial R2
ENZYMES
Lipase
> 1000
IU/L
Peroxydase (POD)
> 1700
IU/L
Glycerol 3 phosphate oxydase (GPO) > 3000
IU/L
Glycerol Kinase (GK)
> 660
IU/L
4 - Amino – antipyrine (PAP)
0.5
mmol/L
Adenosine triphosphate Na (ATP)
1.3
mmol/L
Vial R3
STANDARD
Glycerol
2.28
mmol/L
Equivalent to trioleine or triglycerides 200 mg/dL (2.28 mmol/L)
(4) (5)
Fossati and Prencipe method associated with Trinder reaction.
Reaction scheme is as follows:
Lipase
Triglycerides
Glycerol + free fatty acids
GK
Glycerol + ATP
Glycerol 3 Phosphate + ADP
GPO
Glycerol 3 Phosphate +
O2
Dihydroxyacetone
GPO
Phosphate +
H2O2
POD
H2O2
+ 4
Chlorophenol + PAP
Quinoneimine H2O2 + 4-Chlorophenol + PAP (pink) +
H2O