This study aimed to detect of methicillin resistant
S. aureus
(MRSA) which caused neonatal infections and to
detect the genes being responsible of antibiotic re
sistance in these bacteria by molecular technique (
PCR). Antibiotic
sensitivity test results demonstrated that,
S. aureus
isolates were highly resistant against most antibi
otics, 100% for each
AMP, AMC, CTX, CTR, CAZ, KF and K. whereas it s res
istance
?
70% for most other antibiotics. While 0% resistance
(100% sensitivity) was shown in each IPM, MRP. The
results of minimum inhibitory concentration MIC by
using HiComb
test showed that
S. aureus
has 100% resistance for both MET and OX with MIC v
alues ranged between (5-240
?
g/ml and
32-256
?
g/ml respectively). While 0% resistance reported fo
r VA (0.024-0.512
?
g/ml). PCR results demonstrated that,
among 35 isolates of
S. aurus
were determined previously as MRSA by phenotypic m
ethods, 91.4% have
mecA
gene, 37%
have
nuc gene
and 2.9% have
vanA