Throughout this study, multi-drug resistant P. aeruginosa was isolated from neonatal infections in order to detectthe genes being responsible of antibiotic resistance in these bacteria by molecular technique (PCR). Antibiotic sensitivity test results demonstrated that P. aeruginosa have (100%) resistance against each Carbenicillin, Amoxyclave, Cephalothin and Rifampicin, and high level resistance ?70% for most other antibiotics.While 0% resistance (100% sensitivity) was shown against each IPM, MRP. The results of minimum inhibitory concentration,MIC, by using HiComb test showed that P. aeruginosa has 100% resistance for Amoxyclave (4-240?g/ml), 70% for Cloramphenicol(4-120 ?g/ml), 60% for Cefepem(16-128 ?g/ml), 50% for Ciprofloxacin(0.5-120 ?g/ml), 30% for Amikacin(0.032-128 ?g/ml), 20% for Piperacillin(5-120 ?g/ml) and 10% for Ceftazidime(8-64 ?g/ml). PCR results demonstrated that among 35 isolates of                P. aeruginosa, highest incidence 51.4%have arr-2 gene followed by 49% have aac(3)I and 37%have ^blaCARB.