131-blood samples were collected from neonates who were admitted to the preterm units and Neonatal Intensive
Care Unit (NICU) in Babylon Hospital for Pediatric and Gynecology. Samples were cultured on many different
media, 90(68.7%) of blood samples with positive culture. Among 96 bacterial isolates isolated from these cultures,
40(41.7%) gram negative bacteria versus 56(58.3%) gram positive bacteria. The most frequently isolated bacteria
were 13(32.5%) Klebsiella pneumoniae and 11(27.5%) pseudomonas aeruginosa among gram negative. while
among gram positive staphylococci have highest rate of isolation 39(69.6%) which included 16(41%) coagulase
positive staphylococci and 23(59%) coagulase negative staphylococci (CoNS). Antibiotic susceptibility test
performed by disc diffusion test for 28 antibiotics, most isolates have fully resistance to penicillins and
cephalosporins. While fully sensitivity was shown in each imipenem and meropenem for both gram negative and
gram positive bacteria